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biotinylated horse universal secondary antibody  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated horse universal secondary antibody
    Biotinylated Horse Universal Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 498 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vectastain+universal+secondary+antibody/VECTASTAIN+Universal+Quick+HRP+Kit+(Peroxidase%2C+concentrate)/pm41776092-93-15-21
    Average 94 stars, based on 498 article reviews
    biotinylated horse universal secondary antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: Topical Application of a Dual PI3K/mTOR Inhibitor Prevents Anal Carcinogenesis in an HPV Mouse Model of Anal Cancer
    Article Snippet: Sections were then stained with a monoclonal rabbit antibody against pAkt (1:50 in 5% horse serum in PBS; antibody #3787, Cell Signaling Technology) or a polyclonal rabbit antibody against pS6 (1:50 in 5% horse serum in PBS; antibody #2211, Cell Signaling Technology) overnight at 4° Celsius. .. Each slide was then washed and incubated with VectaStain universal secondary antibody (1:50 in 5% horse serum in PBS; Vector Laboratories). ..

    Article Title: Efficacy of Topically Administered Dihydroartemisinin in Treating Papillomavirus-Induced Anogenital Dysplasia in Preclinical Mouse Models.
    Article Snippet: Sections were then stained with either human papillomavirus type 16/18 E6 antibody (C1P5) (Cat No. GTX60410, GeneTex, Inc., Irvine, CA, USA; 1:250 in 5% horse serum with phosphate-buffered saline (PBS)) or human papillomavirus type 16 E7 antibody [6F3] (Cat No. GTX20070, GeneTex, Inc., Irvine, CA, USA; 1:500 in 5% horse serum with phosphate-buffered saline (PBS)) overnight at 4 ◦C in a humidified chamber. .. Each slide was then washed with 1× PBS and incubated with VectaStain universal secondary antibody (Cat No. BP-1400-50, Vector Laboratories, Burlingame, CA, USA, 1:50 in 5% horse serum in PBS;), followed by VectaStain Elite ABC reagent (R.T.U.) (Cat No. PK-7100, Vector Laboratories, Burlingame, CA, USA) as per the manufacturer’s protocol. .. Finally, sections were incubated with 3,3′-diaminobenzidine substrate (DAB Peroxidase (HRP) Substrate; Cat No. SK-4100, Vector Laboratories, Burlingame, CA, USA), counterstained with hematoxylin, dehydrated, and mounted.

    Article Title: Efficacy of Topically Administered Dihydroartemisinin in Treating Papillomavirus-Induced Anogenital Dysplasia in Preclinical Mouse Models
    Article Snippet: Sections were then stained with either human papillomavirus type 16/18 E6 antibody (C1P5) (Cat No. GTX60410, GeneTex, Inc., Irvine, CA, USA; 1:250 in 5% horse serum with phosphate-buffered saline (PBS)) or human papillomavirus type 16 E7 antibody [6F3] (Cat No. GTX20070, GeneTex, Inc., Irvine, CA, USA; 1:500 in 5% horse serum with phosphate-buffered saline (PBS)) overnight at 4 °C in a humidified chamber. .. Each slide was then washed with 1× PBS and incubated with VectaStain universal secondary antibody (Cat No. BP-1400-50, Vector Laboratories, Burlingame, CA, USA, 1:50 in 5% horse serum in PBS;), followed by VectaStain Elite ABC reagent (R.T.U.) (Cat No. PK-7100, Vector Laboratories, Burlingame, CA, USA) as per the manufacturer’s protocol. .. Finally, sections were incubated with 3,3′-diaminobenzidine substrate (DAB Peroxidase (HRP) Substrate; Cat No. SK-4100, Vector Laboratories, Burlingame, CA, USA), counterstained with hematoxylin, dehydrated, and mounted.

    Article Title: Systemic Delivery of a Dual PI3K/mTOR Inhibitor More Effective than Topical Delivery in Preventing Anal Carcinogenesis in an HPV Transgenic Mouse Model
    Article Snippet: Sections were then stained with a monoclonal rabbit antibody against pAKT serine 473 (1:50 in 5% horse serum in PBS, antibody #3787; Cell Signaling Technology, Danvers, Massachusetts, USA) or a polyclonal rabbit antibody against pS6 ribosomal protein Ser235/236 (1:50 in 5% horse serum in PBS, antibody #2211; Cell Signaling Technology, Danvers, MA, USA) overnight at 4°C. .. Each slide was then washed and incubated with VectaStain universal secondary antibody (1:50 in 5% horse serum in PBS; Vector Laboratories, Inc., Burlingame, California, USA). ..

    Article Title: PI3K/mTOR inhibition prevents anal cancer in mice with established low-grade anal dysplasia.
    Article Snippet: Sections were then stained with a monoclonal rabbit antibody against pAKT (1:50 in 5% horse serum in phosphate buffered saline (PBS); antibody #3787, Cell Signaling Technology) or a polyclonal rabbit antibody against pS6 (1:50 in 5% horse serum in PBS; antibody #2211, Cell Signaling Technology) overnight at 4◦ Celsius. .. Each slide was then washed and incubated with VectaStain universal secondary antibody (1:50 in 5% horse serum in PBS; Vector Laboratories). ..



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